We show that 12 loci originally identified in Sassafras randaiense are polymorphic microsatellites in Sassafras albidum and can be used to genotype individuals which belong to the same clone. Microsatellite markers provide a cost-effective approach at unambiguously differentiating clones. Most temperate plant species have some form of clonal growth. While clonal growth provides advantages in favorable, stable environments (such as increased foraging and reproductive assurance), it does not provide offspring with the same level of genetic variability for adaptation to changes in the environment as sexual reproduction. Sassafras albidum has been characterized as producing clones from its roots, but no studies have described the clonal growth of S. albidum in natural conditions. These microsatellite markers will be used to determine individuals and the fine scale spatial clonal structure of S. albidum on the Domain. The sampling strategy involved both random samples from throughout the Domain and nested plot sampling from Dr. Jonathan Evans’ long term demographic plot, which will enable the identification of the spatial scale relevant for clonal structure in S. albidum.